A proteomic approach to determine AM symbiosis associated alterations in root cortical cells of Medicago truncatula

Nicole Gaude¹, Waltraud X. Schulze¹, Philip Franken² and Franziska Krajinski¹
¹Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm, Germany;
²Institute of Vegetable and Ornamental Crops, Theodor-Echtermeyer-Weg 1, 14979 Großbeeren

The arbuscular mycorrhiza symbiosis (AMS) formed between Medicago truncatula and the fungal partner Glomus intraradices is a well established model system to investigate molecular mechanisms taking place during the interaction of both symbiotic partners. The analysis of reprogramming processes is challenging because of the non-synchronous colonization of the root system by the fungal partner. To dissect developmental stages of the AMS, we established an experimental system with a high cellular resolution for analyzing proteome alterations to provide a comprehensive insight into cell type specific protein regulation.
A dataset analyzing cell specific proteome alterations of arbuscule containing cortical cells of M. truncatula roots colonized by G. intraradices (arb) versus cortex cells of non-mycorrhizal M. truncatula roots (cor) was generated by coupling laser capture microdissection (LCM) and liquid chromatography-tandem mass spectrometry (LC/MS/MS) as an analytical tool. Using this highly specific approach, 500 proteins were identified in root cortical cells. Among them, 99 proteins were determined as fungal proteins, and 188 plant proteins could be detected specifically accumulating in arbuscule containing root cortical cells. Furthermore, these data point to a specific accumulation of distinct transporters, stress related proteins and proteins involved in lipid metabolism associated with the arbuscule formation. In summary, this large scale analysis highlights the accumulation of AM symbiosis responsive proteins specifically in arbuscule containing cells for the first time.

Files:

  1. Exclusively expressed proteins in Medicago truncatula arbuscule containing root cortical cells
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  2. Differentially expressed proteins in arbuscule containing cells relative to non-mycorrhizal root cortical cells
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  3. Exclusively expressed proteins in cortical cells of non-mycorrhizal roots
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  4. emPAI of fungi-specific proteins in arbuscule containing root cortical cells
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